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primer extension system-amv reverse transcriptase  (Promega)

 
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    Structured Review

    Promega primer extension system-amv reverse transcriptase
    Primer Extension System Amv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amv+reverse+transcriptase+primer+extension+system/amv+reverse+transcription+kit/pmc03050692-84-12-17
    Average 90 stars, based on 1 article reviews
    primer extension system-amv reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Control of gdhR Expression in Neisseria gonorrhoeae via Autoregulation and a Master Repressor (MtrR) of a Drug Efflux Pump Operon
    Article Snippet: .. The AMV reverse transcriptase primer extension system from Promega (Madison, WI) was used as described by the manufacturer. ..

    Article Title: Streptococcus mutans NADH Oxidase Lies at the Intersection of Overlapping Regulons Controlled by Oxygen and NAD + Levels
    Article Snippet: .. Primer extension analysis of 50 μg total RNA was performed using the Promega AMV Reverse Transcriptase Primer Extension System (Promega, Madison, WI). .. The primer nox pe-F1 ( ) (10 pmol) was end labeled with [γ- 32 P]ATP (6,000 Ci/mmol; PerkinElmer, Boston, MA) and T4 polynucleotide kinase (PNK) (Life Technologies, Carlsbad, CA).

    Article Title: Transcription and genetic analyses of a putative N -acetylmuramyl- L -alanine amidase in Borrelia burgdorferi
    Article Snippet: Further purification of RNA samples was performed using a Qiagen RNA isolation kit. .. The AMV Reverse Transcriptase Primer Extension System (Promega) was used to generate cDNA for RT-PCR or for primer extension analysis. table ft1 table-wrap mode=article t1 caption a4 Oligonucelotide primers used in this study Primer extension analysis was performed as before with slight modification ( Ge et al. , 1997a , b ). .. Briefly, 20 pmol of the primer P15 ( ) was labeled with 32 P-ATP at 37 °C for 30 min and purified with a Qiagen Nucleotide Removal Kit.

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli
    Article Snippet: .. Primer extension reactions were carried out on 8 μg of total RNA using the AMV reverse transcriptase primer extension system according to manufacturer’s instructions (Promega). ..

    Article Title: Functional Expression of the Murine Connexin 36 Gene Coding for a Neuron-Specific Gap Junctional Protein
    Article Snippet: Primer Extension Analysis Poly(A) + RNA was isolated from mouse brain RNA ( see above ) using an mRNA purification kit (Amersham Pharmacia Biotech, Freiburg, Germany). .. Cx36 specific primers Pex1 (position −458 to −478 with respect to the start codon ATG), and Pex2 (position −363 to −394) were phosphorylated with [γ- 32 P] ATP to a specific activity of 2 × 10 7 cpm/μg using an AMV reverse transcriptase primer extension system (Promega). ..

    Article Title: The Extracytoplasmic Function Sigma Factor σ S Protects against both Intracellular and Extracytoplasmic Stresses in Staphylococcus aureus
    Article Snippet: .. Primer extension analysis was carried out as described previously 156 (62) using the AMV reverse transcriptase primer extension system (Promega) according to the 157 manufacturer’s guidelines. .. RNA for primer extension reactions was extracted using an RNeasy 158 kit (Qiagen) as described previously (35).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Transcription and genetic analyses of a putative N -acetylmuramyl- L -alanine amidase in Borrelia burgdorferi
    Article Snippet: Further purification of RNA samples was performed using a Qiagen RNA isolation kit. .. The AMV Reverse Transcriptase Primer Extension System (Promega) was used to generate cDNA for RT-PCR or for primer extension analysis. table ft1 table-wrap mode=article t1 caption a4 Oligonucelotide primers used in this study Primer extension analysis was performed as before with slight modification ( Ge et al. , 1997a , b ). .. Briefly, 20 pmol of the primer P15 ( ) was labeled with 32 P-ATP at 37 °C for 30 min and purified with a Qiagen Nucleotide Removal Kit.

    Modification:

    Article Title: Transcription and genetic analyses of a putative N -acetylmuramyl- L -alanine amidase in Borrelia burgdorferi
    Article Snippet: Further purification of RNA samples was performed using a Qiagen RNA isolation kit. .. The AMV Reverse Transcriptase Primer Extension System (Promega) was used to generate cDNA for RT-PCR or for primer extension analysis. table ft1 table-wrap mode=article t1 caption a4 Oligonucelotide primers used in this study Primer extension analysis was performed as before with slight modification ( Ge et al. , 1997a , b ). .. Briefly, 20 pmol of the primer P15 ( ) was labeled with 32 P-ATP at 37 °C for 30 min and purified with a Qiagen Nucleotide Removal Kit.

    Activity Assay:

    Article Title: Functional Expression of the Murine Connexin 36 Gene Coding for a Neuron-Specific Gap Junctional Protein
    Article Snippet: Primer Extension Analysis Poly(A) + RNA was isolated from mouse brain RNA ( see above ) using an mRNA purification kit (Amersham Pharmacia Biotech, Freiburg, Germany). .. Cx36 specific primers Pex1 (position −458 to −478 with respect to the start codon ATG), and Pex2 (position −363 to −394) were phosphorylated with [γ- 32 P] ATP to a specific activity of 2 × 10 7 cpm/μg using an AMV reverse transcriptase primer extension system (Promega). ..

    other:

    Article Title: Streptococcus mutans NADH Oxidase Lies at the Intersection of Overlapping Regulons Controlled by Oxygen and NAD + Levels
    Article Snippet: The primer noxpe-F1 (Table 2) (10 pmol) was end labeled with [ -32P]ATP (6,000 Ci/mmol; PerkinElmer, Boston, MA) and T4 polynucleotide kinase (PNK) (Life Technologies, Carlsbad, CA).



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